Automated Polysome Profile Aligner

October 05, 2017

[Written in R]

Takes multiple polysome profiles, identifies a conserved location within the profiles, then rescales the x-axis to align all profiles based on that location. Profile inputs must be .csv files containing 'Absorbance' and 'Distance Through Gradient' information (trims first 32 lines of data files for streamlined use with BioComp data files, can be commented out if necessary). Default specifications align profiles based on the x-axis coordinate where the absorbance crosses a threshold value for the second time (currently set to 3/4 of the absorbance maximum value, which locates the decrease in signal from non-ribosome-bound RNA at the top of the gradient).

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